Anyone who knows anything about Gus Fring knows that he has nothing but disdain for Hector Salamanca (Mark Margolis). Hector was the former right-hand man to Don Eladio before being confined to a wheelchair, and the two have a deeply contentious history.
In the present day, Fring frequently visits Hector in his nursing home, seemingly with the intention of taunting him. During his visits to the nursing home, Gus asks Hector to look at him, and Hector always refuses. Gus wants Hector to admit defeat in their years-long war, but Hector won't allow Gus to have the victory.
In one blooper from Season 4, Gus asks Hector, "Is today the day?" Margolis doesn't seem to be totally ready for the scene, though, and instead is on the verge of laughing. So, instead, of proceeding with the scene, Esposito simply says, "I guess today's the day," and they both break into a hearty chuckle.
The moment is striking not just because it's strange to see Gus and Hector laughing but also because it's strange to see these two characters laughing together. Gus and Hector are the opposite of friends, but that doesn't mean the actors playing them are also mortal enemies.